A Putative Zn₂Cys₆ Transcription Factor Is Associated With Isoprothiolane Resistance in Magnaporthe oryzae.

ABSTRACT: Isoprothiolane (IPT), a systemic fungicide, has been applied to control rice blast since the 1970s. Although resistance to IPT has been observed, the mechanism of resistance still has not been fully elucidated. In this study, nucleotide polymorphisms were detected between two IPT-resistant mutants generated in the lab, and their parental wild type isolates using a whole-genome sequencing approach. In the genomes of the two resistant mutants, single point mutations were identified in a gene encoding a Zn₂Cys₆ transcription factor-like protein. Notably, either knocking out the gene or replacing the wild type allele with the mutant allele (R343W) in a wild type isolate resulted in resistance to IPT, indicating that the gene is associated with IPT resistance, and thus was

SUBMITTER: Wang ZQ 

PROVIDER: S-EPMC6220061 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature