Integrating impedance-based growth-rate monitoring into a microfluidic cell culture platform for live-cell microscopy.

ABSTRACT: Growth rate is a widely studied parameter for various cell-based biological studies. Growth rates of cell populations can be monitored in chemostats and micro-chemostats, where nutrients are continuously replenished. Here, we present an integrated microfluidic platform that enables long-term culturing of non-adherent cells as well as parallel and mutually independent continuous monitoring of (i) growth rates of cells by means of impedance measurements and of (ii) specific other cellular events by means of high-resolution optical or fluorescence microscopy. Yeast colonies were grown in a monolayer under culturing pads, which enabled high-resolution microscopy, as all cells were in the same focal plane. Upon cell growth and division, cells leaving the culturing area passed over a pair of ele

SUBMITTER: Chawla K 

PROVIDER: S-EPMC6220194 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature