Positive effect of RORγt on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto's thyroiditis.

ABSTRACT:

Objectives

To investigate the positive effect of Th17 cells on the prognosis of patients with PTC and HT.

Methods

The expression of nuclear specific marker RORγt of Th17 cells in fresh and paraffin thyroid tissues and serum specimens were analyzed. Flow cytometry was used to detect the formation rates of Th17 cells (CD3⁺CD8^-IL-17A⁺/CD3⁺CD8^-%) at different time points after co-culture of thyroid papillary carcinoma cell line (TPC-1 and K1) and umbilical cord blood initial T lymphocytes. The protein expression of RORγt in T lymphocytes after co-culture was detected. Preoperative serum levels of Th17 (IL-17) cytokines were measured.

Results

The positive expression of RORγt in the tumor microenvironment of PTC patient

SUBMITTER: Zeng R 

PROVIDER: S-EPMC6220227 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature