A versatile method for the UVA-induced cross-linking of acetophenone- or benzophenone-functionalized DNA.

ABSTRACT: Bioconjugation, biosensing, bioimaging, bionanomaterials, etc., are only a few examples of application of functionalized DNA. Since base-modified nucleic acids contribute not only to a broad range of biotechnological fields but also to the understanding of various cellular processes, it is crucial to design novel modifications with unique properties. Here, we demonstrate the utilization of N⁴-cytidine modified oligonucleotides, which contain reactive acetophenone (AP) or benzophenone (BP) groups, for the UV-induced cross-linking. We find that terminal deoxynucleotidyl transferase-mediated 3'-tailing using AP/BP-containing modified nucleotides generates photoactive DNA, suitable for a straightforward covalent cross-linking with both interacting proteins and a variety of well-know

SUBMITTER: Jakubovska J 

PROVIDER: S-EPMC6220319 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature