Accurate, Sensitive, and Precise Multiplexed Proteomics Using the Complement Reporter Ion Cluster.

ABSTRACT: Quantitative analysis of proteomes across multiple time points, organelles, and perturbations is essential for understanding both fundamental biology and disease states. The development of isobaric tags (e.g., TMT) has enabled the simultaneous measurement of peptide abundances across several different conditions. These multiplexed approaches are promising in principle because of advantages in throughput and measurement quality. However, in practice, existing multiplexing approaches suffer from key limitations. In its simple implementation (TMT-MS2), measurements are distorted by chemical noise leading to poor measurement accuracy. The current state-of-the-art (TMT-MS3) addresses this but requires specialized quadrupole-iontrap-Orbitrap instrumentation. The complement reporter ion approach

SUBMITTER: Sonnett M 

PROVIDER: S-EPMC6220677 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature