Resemblance of the human liver sinusoid in a fluidic device with biomedical and pharmaceutical applications.

ABSTRACT: Maintenance of the complex phenotype of primary hepatocytes in vitro represents a limitation for developing liver support systems and reliable tools for biomedical research and drug screening. We herein aimed at developing a biosystem able to preserve human and rodent hepatocytes phenotype in vitro based on the main characteristics of the liver sinusoid: unique cellular architecture, endothelial biodynamic stimulation, and parenchymal zonation. Primary hepatocytes and liver sinusoidal endothelial cells (LSEC) were isolated from control and cirrhotic human or control rat livers and cultured in conventional in vitro platforms or within our liver-resembling device. Hepatocytes phenotype, function, and response to hepatotoxic drugs were analyzed. Results evidenced that mimicking the in vivo si

SUBMITTER: Ortega-Ribera M 

PROVIDER: S-EPMC6220781 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature