Gating control and K⁺ uptake by the KAT1 K⁺ channel leaveraged through membrane anchoring of the trafficking protein SYP121.

ABSTRACT: Vesicle traffic is tightly coordinated with ion transport for plant cell expansion through physical interactions between subsets of vesicle-trafficking (so-called SNARE) proteins and plasma membrane Kv channels, including the archetypal inward-rectifying K⁺ channel, KAT1 of Arabidopsis. Ion channels open and close rapidly over milliseconds, whereas vesicle fusion events require many seconds. Binding has been mapped to conserved motifs of both the Kv channels and the SNAREs, but knowledge of the temporal kinetics of their interactions, especially as it might relate to channel gating and its coordination with vesicle fusion remains unclear. Here, we report that the SNARE SYP121 promotes KAT1 gating through a persistent interaction that alters the stability of the channel, both in

SUBMITTER: Lefoulon C 

PROVIDER: S-EPMC6220998 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature