Pulmonary metastatic colonisation and granulomas in NOX2-deficient mice.

ABSTRACT: Metastasis is the leading cause of death in cancer patients, and successful colonisation of a secondary organ by circulating tumour cells (CTCs) is the rate-limiting step of this process. We used tail-vein injection of B16-F10 melanoma cells into mice to mimic the presence of CTCs and to allow for the assessment of host (microenvironmental) factors that regulate pulmonary metastatic colonisation. We found that mice deficient for the individual subunits of the NADPH oxidase of myeloid cells, NOX2 (encoded by Cyba, Cybb, Ncf1, Ncf2, and Ncf4), all showed decreased pulmonary metastatic colonisation. To understand the role of NOX2 in controlling tumour cell survival in the pulmonary microenvironment, we focused on Cyba-deficient (Cyba^tm1a ) mice, which showed the most significant de

SUBMITTER: van der Weyden L 

PROVIDER: S-EPMC6221033 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature