Flow immunophenotyping of benign lymph nodes sampled by FNA: Representative with diagnostic pitfalls.

ABSTRACT:

Background

Fine-needle aspiration with flow cytometry (FNA-FC) is routinely used in the evaluation of lymph nodes suspicious for lymphoma, yet data comparing immunophenotype distributions and outliers in benign lymph nodes sampled by fine-needle aspiration (FNA) versus excision are lacking.

Methods

Flow cytometry data from 289 benign lymph node FNA cases were assessed for the overall antigen distribution, with a focus on outliers relevant to the diagnosis of lymphoma. Distributions and outlier proportions were compared with those of a separate cohort of 298 excisional biopsies.

Results

Compared with excisional biopsies, FNA specimens overrepresented CD3+ events (72% vs 63%), underrepresented CD19+ events (22% vs 29%), and had 25% fewer large cell-gated events. Normali

SUBMITTER: Scott GD 

PROVIDER: S-EPMC6221160 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature