The p53 C terminus controls site-specific DNA binding and promotes structural changes within the central DNA binding domain.

ABSTRACT: DNA binding by numerous transcription factors including the p53 tumor suppressor protein constitutes a vital early step in transcriptional activation. While the role of the central core DNA binding domain (DBD) of p53 in site-specific DNA binding has been established, the contribution of the sequence-independent C-terminal domain (CTD) is still not well understood. We investigated the DNA-binding properties of a series of p53 CTD variants using a combination of in vitro biochemical analyses and in vivo binding experiments. Our results provide several unanticipated and interconnected findings. First, the CTD enables DNA binding in a sequence-dependent manner that is drastically altered by either its modification or deletion. Second, dependence on the CTD correlates with the extent to which

SUBMITTER: Laptenko O 

PROVIDER: S-EPMC6221458 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature