TET2 facilitates PPARγ agonist-mediated gene regulation and insulin sensitization in adipocytes.

ABSTRACT: Emerging evidence indicates that epigenetic mechanisms like DNA methylation directly contribute to metabolic regulation. For example, we previously demonstrated that de novo DNA methyltransferase Dnmt3a plays a causal role in the development of adipocyte insulin resistance. Recent studies suggest that DNA demethylation plays an important role in the developmental process of adipocytes. However, little is known about whether DNA demethylase ten-eleven translocation (TET) proteins regulate the metabolic functions of adipocytes.

Methods

The expression of Tet genes was assessed in the fractionated adipocytes of chow- and high fat diet-fed C57/Bl6 mice using qPCR and western blotting. The effect of Tet2 gain- or loss-of-function in fully mature 3T3-L1 adipocytes in the presence/absence o

SUBMITTER: Bian F 

PROVIDER: S-EPMC6221917 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature