Plasma membrane profiling during enterohemorrhagic <i>E. coli</i> infection reveals that the metalloprotease StcE cleaves CD55 from host epithelial surfaces.

ABSTRACT: Enterohemorrhagic Escherichia coli (EHEC) is one of several E. coli pathotypes that infect the intestinal tract and cause disease. Formation of the characteristic attaching and effacing lesion on the surface of infected cells causes significant remodeling of the host cell surface; however, limited information is available about changes at the protein level. Here we employed plasma membrane profiling, a quantitative cell-surface proteomics technique, to identify host proteins whose cell-surface levels are altered during infection. Using this method, we quantified more than 1100 proteins, 280 of which showed altered cell-surface levels after exposure to EHEC. 22 host proteins were significantly reduced on the surface of infected epithelial cells. These included both known and u

SUBMITTER: Furniss RCD 

PROVIDER: S-EPMC6222108 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature