Assembly Order of Flagellar Rod Subunits in Bacillus subtilis.

ABSTRACT: Bacterial flagella contain an axle-like rod that transits the cell envelope and connects the transmembrane basal body to the extracellular hook and filament. Although the rod is a crucial component of the flagellum, its structure and assembly are poorly understood. Previous reports defining the order of rod assembly in Gram-negative bacteria suggest that the rod requires five proteins to successfully assemble, but assembly intermediates have not been well characterized due to metastability and periplasmic proteolysis. Bacillus subtilis is a Gram-positive, genetically tractable model bacterium that synthesizes flagella and lacks a true periplasm. Here, we genetically, biochemically, and cytologically determine the assembly order of the flagellar rod proteins from cell proximal to distal as

SUBMITTER: Burrage AM 

PROVIDER: S-EPMC6222195 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature