Characterizing the Circulating Cell Populations in Traumatic Heterotopic Ossification.

ABSTRACT: Heterotopic ossification (HO) occurs secondary to trauma, causing pain and functional limitations. Identification of the cells that contribute to HO is critical to the development of therapies. Given that innate immune cells and mesenchymal stem cells are known contributors to HO, we sought to define the contribution of these populations to HO and to identify what, if any, contribution circulating populations have to HO. A shared circulation was obtained using a parabiosis model, established between an enhanced green fluorescent protein-positive/luciferase⁺ donor and a same-strain nonreporter recipient mouse. The nonreporter mouse received Achilles tendon transection and dorsal burn injury to induce HO formation. Bioluminescence imaging and immunostaining were performed to defin

SUBMITTER: Loder SJ 

PROVIDER: S-EPMC6222270 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature