Epigenetic-scale comparison of human iPSCs generated by retrovirus, Sendai virus or episomal vectors.

ABSTRACT: Human induced pluripotent stem cells (iPSCs) are established by introducing several reprogramming factors, such as OCT3/4, SOX2, KLF4, c-MYC. Because of their pluripotency and immortality, iPSCs are considered to be a powerful tool for regenerative medicine. To date, iPSCs have been established all over the world by various gene delivery methods. All methods induced high-quality iPSCs, but epigenetic analysis of abnormalities derived from differences in the gene delivery methods has not yet been performed. Here, we generated genetically matched human iPSCs from menstrual blood cells by using three kinds of vectors, i.e., retrovirus, Sendai virus, and episomal vectors, and compared genome-wide DNA methylation profiles among them. Although comparison of aberrant methylation revealed t

SUBMITTER: Nishino K 

PROVIDER: S-EPMC6222281 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature