Converting Galactose into the Rare Sugar Talose with Cellobiose 2-Epimerase as Biocatalyst.

ABSTRACT: Cellobiose 2-epimerase from Rhodothermus marinus (RmCE) reversibly converts a glucose residue to a mannose residue at the reducing end of β-1,4-linked oligosaccharides. In this study, the monosaccharide specificity of RmCE has been mapped and the synthesis of d-talose from d-galactose was discovered, a reaction not yet known to occur in nature. Moreover, the conversion is industrially relevant, as talose and its derivatives have been reported to possess important antimicrobial and anti-inflammatory properties. As the enzyme also catalyzes the keto-aldo isomerization of galactose to tagatose as a minor side reaction, the purity of talose was found to decrease over time. After process optimization, 23 g/L of talose could be obtained with a product purity of 86% and a yie

SUBMITTER: Van Overtveldt S 

PROVIDER: S-EPMC6222537 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature