Arginase II Contributes to the Ca²⁺/CaMKII/eNOS Axis by Regulating Ca²⁺ Concentration Between the Cytosol and Mitochondria in a p32-Dependent Manner.

ABSTRACT: Background Arginase II activity contributes to reciprocal regulation of endothelial nitric oxide synthase ( eNOS ). We tested the hypotheses that arginase II activity participates in the regulation of Ca²⁺/Ca²⁺/calmodulin-dependent kinase II / eNOS activation, and this process is dependent on mitochondrial p32. Methods and Results Downregulation of arginase II increased the concentration of cytosolic Ca²⁺ ([Ca²⁺]c) and decreased mitochondrial Ca²⁺ ([Ca²⁺]m) in microscopic and fluorescence-activated cell sorting analyses, resulting in augmented eNOS Ser1177 phosphorylation and decreased eNOS Thr495 phosphorylation through Ca²⁺/Ca²⁺/calmodulin-dependent kinase II . These changes were observed in human umb

SUBMITTER: Koo BH 

PROVIDER: S-EPMC6222941 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature