Microscopy with ultraviolet surface excitation for rapid slide-free histology.

ABSTRACT: Histological examination of tissues is central to the diagnosis and management of neoplasms and many other diseases and is a foundational technique for preclinical and basic research. However, commonly used bright-field microscopy requires prior preparation of micrometre-thick tissue sections mounted on glass slides-a process that can require hours or days, contributes to cost and delays access to critical information. Here, we introduce a simple, non-destructive slide-free technique that, within minutes, provides high-resolution diagnostic histological images resembling those obtained from conventional haematoxylin and eosin histology. The approach, which we named microscopy with ultraviolet surface excitation (MUSE), can also generate shape and colour-contrast information. MUSE relies on

SUBMITTER: Fereidouni F 

PROVIDER: S-EPMC6223324 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature