Expression analysis of two SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) genes during in vitro morphogenesis in Spanish cedar (Cedrela odorata L.).

ABSTRACT: Somatic embryogenesis (SE) is one of the most important steps during regeneration, but the molecular mechanism of SE remains unclear for Cedrela odorata. SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) is one of the genes associated with induction of SE and is considered a marker of cells competent to form somatic embryos. Our objective was to clone and characterize the SERK1 and SERK2 gene homologues and analyze their expression patterns during in vitro morphogenesis in Spanish cedar. CoSERK1 and CoSERK2 were isolated from cedar, both share domains characteristic of the SERK family, including leucine-rich repeats, a proline-rich motif, a transmembrane domain, and kinase domains. Embryogenic cultures were established from callus cultures induced

SUBMITTER: Porras-Murillo R 

PROVIDER: S-EPMC6223411 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature