Optimising collagen scaffold architecture for enhanced periodontal ligament fibroblast migration.

ABSTRACT: Design of cell-free scaffolds for endogenous cell recruitment requires an intimate knowledge of precise relationships between structure and biological function. Here, we use morphological analysis by Micro-CT to identify the key structural features necessary for periodontal ligament fibroblast recruitment into collagen scaffolds. By the combined use of time-lapse imaging and end-point invasion analysis, we distinguish the influences of pore size, pore wall alignment, and pore transport pathways (percolation diameter) on the individual cell migration and bulk invasion characteristics of these fibroblasts. Whereas maximising percolation diameter increased individual cell speed, elongation and directionality, and produced the most rapid bulk cell invasion, a pore size of 100 μm was found to b

SUBMITTER: Ashworth JC 

PROVIDER: S-EPMC6223802 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature