AFLP-AFLP in silico-NGS approach reveals polymorphisms in repetitive elements in the malignant genome.

ABSTRACT: The increasing interest in exploring the human genome and identifying genetic risk factors contributing to the susceptibility to and outcome of diseases has supported the rapid development of genome-wide techniques. However, the large amount of obtained data requires extensive bioinformatics analysis. In this work, we established an approach combining amplified fragment length polymorphism (AFLP), AFLP in silico and next generation sequencing (NGS) methods to map the malignant genome of patients with chronic myeloid leukemia. We compared the unique DNA fingerprints of patients generated by the AFLP technique approach with those of healthy donors to identify AFLP markers associated with the disease and/or the response to treatment with imatinib, a tyrosine kinase inhibitor. Among the statis

SUBMITTER: Koblihova J 

PROVIDER: S-EPMC6224067 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature