Nuclear Nox4 interaction with prelamin A is associated with nuclear redox control of stem cell aging.

ABSTRACT: Mesenchymal stem cells have emerged as an important tool that can be used for tissue regeneration thanks to their easy preparation, differentiation potential and immunomodulatory activity. However, an extensive culture of stem cells in vitro prior to clinical use can lead to oxidative stress that can modulate different stem cells properties, such as self-renewal, proliferation, differentiation and senescence. The aim of this study was to investigate the aging process occurring during in vitro expansion of stem cells, obtained from amniotic fluids (AFSC) at similar gestational age.The analysis of 21 AFSC samples allowed to classify them in groups with different levels of stemness properties. In summary, the expression of pluripotency genes and the proliferation rate were inver

SUBMITTER: Casciaro F 

PROVIDER: S-EPMC6224265 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature