Generation of brilliant green fluorescent petunia plants by using a new and potent fluorescent protein transgene.

ABSTRACT: The application of fluorescent proteins in ornamental plants has lagged behind despite the recent development of powerful genetic tools. Although we previously generated transgenic torenia plants expressing green fluorescent protein from marine plankton (CpYGFP), in which bright fluorescence was easily visible at the whole plant level, the maximum excitation of this protein within the visible light spectrum required the use of a coloured emission filter to eliminate exciting light. Here, to overcome this limitation, we generated transgenic petunia plants expressing eYGFPuv, a CpYGFP derivative exhibiting bright fluorescence under invisible ultraviolet (UV) light excitation, with a novel combination of transcriptional terminator plus translational enhancer. As expected, all transgenic plant

SUBMITTER: Chin DP 

PROVIDER: S-EPMC6224394 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature