Inducing controlled cell cycle arrest and re-entry during asexual proliferation of Plasmodium falciparum malaria parasites.

ABSTRACT: The life cycle of the malaria parasite Plasmodium falciparum is tightly regulated, oscillating between stages of intense proliferation and quiescence. Cyclic 48-hour asexual replication of Plasmodium is markedly different from cell division in higher eukaryotes, and mechanistically poorly understood. Here, we report tight synchronisation of malaria parasites during the early phases of the cell cycle by exposure to DL-α-difluoromethylornithine (DFMO), which results in the depletion of polyamines. This induces an inescapable cell cycle arrest in G₁ (~15 hours post-invasion) by blocking G₁/S transition. Cell cycle-arrested parasites enter a quiescent G₀-like state but, upon addition of exogenous polyamines, re-initiate their cell cycle. This ability to halt ma

SUBMITTER: van Biljon R 

PROVIDER: S-EPMC6224408 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature