Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6).

ABSTRACT: Iron is a very important nutrient for cells; however, it could also cause fatal effects because of its capability to trigger oxidative stress. Due to high exposure to iron from their blood diet, ticks make use of several mechanisms to cope up with oxidative stress. One mechanism is iron sequestration by ferritin and its control protein (IRP). Since the IRP activity is dependent on the ferrous iron concentration, we tried to induce intracellular ferritin (FER1) protein expression by exposing Ixodes scapularis embryo-derived cell line (ISE6) to different concentrations of ferrous sulphate at different time points. We were able to induce FER1 protein after exposure to 2 mM of ferrous sulphate for 48 h, as observed in both Western blotting and indirect immunofluorescent antibody tests. This co

SUBMITTER: Hernandez EP 

PROVIDER: S-EPMC6224502 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature