CD200 facilitates the isolation of corneal epithelial cells derived from human pluripotent stem cells.

ABSTRACT: The in vitro induction of corneal epithelial cells (CECs) from human induced pluripotent stem cells (iPSCs) represents a new strategy for obtaining CE stem/progenitor cells for the surgical reconstruction of a diseased or injured ocular surface. The clinical promise of this strategy is considerable, but if the approaches' potential is to be realised, robust methods for the purification of iPSC-derived CE lineage cells need to be developed to avoid contamination with other cells that may carry the risk of unwanted side effects, such as tumorigenesis. Experiments conducted here revealed that during CEC isolation, CD200-negative selection using a cell sorter considerably reduced the contamination of the cell population with various non-CECs compared with what could be achieved using TRA-1-60,

SUBMITTER: Hayashi R 

PROVIDER: S-EPMC6224558 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature