Dataset Information

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography.

ABSTRACT: Vertebrate models provide indispensable paradigms to study development and disease. Their analysis requires a quantitative morphometric study of the body, organs and tissues. This is often impeded by pigmentation and sample size. X-ray micro-computed tomography (micro-CT) allows high-resolution volumetric tissue analysis, largely independent of sample size and transparency to visual light. Importantly, micro-CT data are inherently quantitative. We report a complete pipeline of high-throughput 3D data acquisition and image analysis, including tissue preparation and contrast enhancement for micro-CT imaging down to cellular resolution, automated data processing and organ or tissue segmentation that is applicable to comparative 3D morphometrics of small vertebrates. Applied to medaka fish, we first create an annotated anatomical atlas of the entire body, including inner organs as a quantitative morphological description of an adult individual. This atlas serves as a reference model for comparative studies. Using isogenic medaka strains we show that comparative 3D morphometrics of individuals permits identification of quantitative strain-specific traits. Thus, our pipeline enables high resolution morphological analysis as a basis for genotype-phenotype association studies of complex genetic traits in vertebrates.

SUBMITTER: Weinhardt V

PROVIDER: S-EPMC6224569 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography.

Weinhardt Venera V Shkarin Roman R Wernet Tobias T Wittbrodt Joachim J Baumbach Tilo T Loosli Felix F

Scientific reports 20181108 1

Vertebrate models provide indispensable paradigms to study development and disease. Their analysis requires a quantitative morphometric study of the body, organs and tissues. This is often impeded by pigmentation and sample size. X-ray micro-computed tomography (micro-CT) allows high-resolution volumetric tissue analysis, largely independent of sample size and transparency to visual light. Importantly, micro-CT data are inherently quantitative. We report a complete pipeline of high-throughput 3D ...[more]

PMID: 30410001

Similar Datasets

Project description:Background and objectivesAnalyzing the anatomical and morphometric information of the hard palate is crucial in prosthodontics, orthodontics, maxillofacial surgery, implant dentistry, and forensic anthropology. No data exist in the literature regarding the morphometric analysis of the hard palate using 3D imaging techniques in Saudi Arabia. Therefore, this study aimed to evaluate the dimensions and types of hard palates using 3D geometric morphometrics and the existence of gender-related differences in a selected Saudi population.Material and methodsThis cross-sectional study was performed by radiological evaluation of arbitrarily collected Cone Beam computed Tomography (CBCT) images includes 130 male and 45 female individuals from our department's archives, radiology department, Dental Hospital, Qassim University. The Galileos® Comfort Plus System (Sirona 3D, Germany) was used to obtain the CBCT images while standardizing the parameters. Images were linearly measured using Galileos software. Measurements of palatal length, palatal breadth, and palatal height, as well as the calculation of the palatine index and palatal height index, were noted and statistically analyzed.ResultsThe mean palatal length, breadth, height, palatine index, and height index were 48.8 ± 3.9 mm, 36.2 ± 3.8 mm, 11.6 ± 2.4 mm, 74.7 ± 8.9, and 32.4 ± 7.1, respectively. The mean palatal length, breadth, and height were greater in men than in women. The majority of the palatine index values corresponded to leptostaphyline (76.6%), followed by mesostaphylline (12%) and brachystaphylline (11.4%), distribution among males and females were not statistical significance (p > 0.05). Palatal height index analysis revealed that the majority were orthostaphyline (59.4%), followed by chamestaphyline (26.3%), and hypsistaphyline (14.3%) these distributions were not significant statistically among males and females(p > 0.05).ConclusionThe palatine index and palatine height index are useful in prosthodontics, orthodontics, and forensic anthropology for identifying races on the basis of the skull.

Dataset Information

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography.

Publications

Quantitative morphometric analysis of adult teleost fish by X-ray computed tomography.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets