The I_Ks Channel Response to cAMP Is Modulated by the KCNE1:KCNQ1 Stoichiometry.

ABSTRACT: The delayed potassium rectifier current, I_Ks, is assembled from tetramers of KCNQ1 and varying numbers of KCNE1 accessory subunits in addition to calmodulin. This channel complex is important in the response of the cardiac action potential to sympathetic stimulation, during which I_Ks is enhanced. This is likely due to channels opening more quickly, more often, and to greater sublevel amplitudes during adrenergic stimulation. KCNQ1 alone is unresponsive to cyclic adenosine monophosphate (cAMP), and thus KCNE1 is required for a functional effect of protein kinase A phosphorylation. Here, we investigate the effect that KCNE1 has on the response to 8-4-chlorophenylthio (CPT)-cAMP, a membrane-permeable cAMP analog, by varying the number of KCNE1 subunits present using fusi

SUBMITTER: Thompson E 

PROVIDER: S-EPMC6224683 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature