Nanoscale Topography and Poroelastic Properties of Model Tissue Breast Gland Basement Membranes.

ABSTRACT: Basement membranes (BMs) are thin layers of condensed extracellular matrix proteins serving as permeability filters, cellular anchoring sites, and barriers against cancer cell invasion. It is believed that their biomechanical properties play a crucial role in determining cellular behavior and response, especially in mechanically active tissues like breast glands. Despite this, so far, relatively little attention has been dedicated to their analysis because of the difficulty of isolating and handling such thin layers of material. Here, we isolated BMs derived from MCF10A spheroids-three-dimensional breast gland model systems mimicking in vitro the most relevant phenotypic characteristics of human breast lobules-and characterized them by atomic force microscopy, enhanced resolution confocal

SUBMITTER: Fabris G 

PROVIDER: S-EPMC6224695 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature