Expression of Bioactive Lunasin Peptide in Transgenic Rice Grains for the Application in Functional Food.

ABSTRACT: Lunasin, a bioactive peptide initially isolated from soybean, has anticancer, anti-inflammatory, and antioxidant activity. Due its great application value, lunasin seems to be a candidate gene in improving the nutritional value of crops. In this study, lunasin was inserted into the rice genome to evaluate whether it was feasible to express lunasin using the rice expression system and improve the bioactivity of protein in rice for our needs. We generatedlunasin-overexpressing rice lines, and chose three independent transgenic rice lines for further study. The lunasin content in trans-lunasin rice detected by UPLC-MS/MS was 1.01 × 10-3 g·kg-1 dry rice flour with grease removal in the lunasin extracts. The antioxidant efficacy of LET (lunasin-enriched fraction from trans-lunasin rice) and PEW

SUBMITTER: Ren G 

PROVIDER: S-EPMC6225134 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature