Quality Analysis of American Ginseng Cultivated in Heilongjiang Using UPLC-ESI^--MRM-MS with Chemometric Methods.

ABSTRACT: American ginseng (Panax quinquefolium) has long been cultivated in China for the function food and medicine. Here, ultra-high performance liquid chromatography was coupled with electrospray ionization and triple quadrupole mass spectrometry (UPLC-ESI^--TQ-MS) for simultaneous detection of 22 ginsenosides in American ginseng cultivated in Mudanjiang district of Heilongjiang. The extraction conditions also were optimized by a Box Behnken design experiment. The optimized result was 31.8 mL/g as ratio of liquid to raw materials, 20.3 min of extraction time, and 235.0 W of extraction powers. The quantitative MS parameters for these 22 compounds were rapidly optimized by single factor experiments employing UPLC-ESI^--multiple reaction monitoring or multiple ion monitor

SUBMITTER: Xia YG 

PROVIDER: S-EPMC6225424 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature