Validation of Internal Control Genes for Quantitative Real-Time PCR Gene Expression Analysis in Morchella.

ABSTRACT: The reliability of qRT-PCR results depend on the stability of reference genes used for normalization, suggesting the necessity of identification of reference genes before gene expression analysis. Morels are edible mushrooms well-known across the world and highly prized by many culinary kitchens. Here, several candidate genes were selected and designed according to the Morchella importuna transcriptome data. The stability of the candidate genes was evaluated with geNorm and NormFinder under three different experimental conditions, and several genes with excellent stability were selected. The extensive adaptability of the selected genes was tested in ten Morchella species. Results from the three experimental conditions revealed that ACT1 and INTF7 were the most prominent genes in Morchella,

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC6225436 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature