Evaluating gut microbiota profiles from archived fecal samples.

ABSTRACT:

Background

Associations between colorectal cancer and microbiota have been identified. Archived fecal samples might be valuable sample sources for investigating causality in carcinogenesis and biomarkers discovery due to the potential of performing longitudinal studies. However, the quality, quantity and stability of the gut microbiota in these fecal samples must be assessed prior to such studies. We evaluated i) cross-contamination during analysis for fecal blood and ii) evaporation in stored perforated fecal immunochemical tests (iFOBT) samples, iii) temperature stability as well as iv) comparison of the gut microbiota diversity and composition in archived, iFOBT and fresh fecal samples in order to assess feasibility of large scale microbiota studies.

Methods

The microbiot

SUBMITTER: Rounge TB 

PROVIDER: S-EPMC6225565 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature