Urothelium with barrier function differentiated from human urine-derived stem cells for potential use in urinary tract reconstruction.

ABSTRACT:

Background

Autologous urothelial cells are often obtained via bladder biopsy to generate the bio-engineered urethra or bladder, while urine-derived stem cells (USC) can be obtained by a non-invasive approach. The objective of this study is to develop an optimal strategy for urothelium with permeability barrier properties using human USC which could be used for tissue repair in the urinary tract system.

Methods

USC were harvested from six healthy adult individuals. To optimize urothelial differentiation, five different differentiation methods were studied. The induced cells were assessed for gene and protein expression markers of urothelial cells via RT-PCR, Western blotting, and immunofluorescent staining. Barrier function and ultrastructure of the tight junction were assess

SUBMITTER: Wan Q 

PROVIDER: S-EPMC6225683 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature