Enhancing enzymatic saccharification of sugarcane bagasse by combinatorial pretreatment and Tween 80.

ABSTRACT:

Background

The recalcitrant structure of lignocellulosic biomass made it challenging for their bioconversion into biofuels and biochemicals. Pretreatment was required to deconstruct the intact structure by the removal of hemicellulose/lignin, improving the cellulose accessibility of enzyme. Combinatorial pretreatments with liquid hot water/H₂SO₄ and ethanol/NaOH of sugarcane bagasse were developed to improve enzymatic hydrolysis under mild conditions.

Results

After one-step 60% ethanol containing 0.5% NaOH pretreatment with solid to liquid ratio of 1/10, the glucose yield after hydrolysis for 72 h with enzyme dosage of 20 FPU/g substrate was enhanced by 41% and 205% compared to that of NaOH or 60% ethanol pretreated solids, respectively. This improvemen

SUBMITTER: Zhang H 

PROVIDER: S-EPMC6225707 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature