Antagonistic interaction between Nodal and insulin modulates pancreatic β-cell proliferation and survival.

ABSTRACT:

Background

Insulin signaling pathway in β-cell is essential to promote β-cells proliferation and survival, while Nodal-ALK7-Smad3 signaling involves β-cells apoptosis. We attempted to address inter-relationship between Nodal and insulin in modulating β-cell proliferation and apoptosis.

Methods

Using INS-1 β-cells and isolated rat islets, we examined the effects of Nodal, insulin, or the two combined on β-cell proliferation and/or apoptosis.

Results

The β-cells under high-glucose or palmitate conditions showed significant up-regulation of Nodal expression and activation of its downstream signaling pathway resulted in increased cleaved caspase-3. Insulin treatment led to significantly attenuated Nodal-induced cell apoptotic pathway. Similar results were found in directl

SUBMITTER: Li J 

PROVIDER: S-EPMC6225724 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature