TRIM32 overexpression improves chemoresistance through regulation of mitochondrial function in non-small-cell lung cancers.

ABSTRACT:

Background

TRIM32 is overexpressed in several human cancers. However, its expression pattern, biological characteristics and mechanisms in human non-small cell lung cancer (NSCLC) have not been reported.

Methods

We examined TRIM32 protein in 115 cases of NSCLC specimens. TRIM32 plasmid transfection and siRNA knockdown was carried out in NSCLC cell lines. AnnexinV/PI and JC-1 staining were performed to examine the change of apoptosis and mitochondrial membrane potential. Western blot was used to detect change of downstream proteins.

Results

We found that TRIM32 protein was upregulated in 69 cases and positively correlated with advanced TNM stage. TRIM32 overexpression also correlated with poor survival of NSCLC patients. Biological assays demonstrated that TRIM32 overe

SUBMITTER: Du Y 

PROVIDER: S-EPMC6225845 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature