Rab18 overexpression promotes proliferation and chemoresistance through regulation of mitochondrial function in human gastric cancer.

ABSTRACT:

Background

Dysregulation of Rab18 has been implicated in human cancers. However, its clinical significance and biological function in gastric cancer have not been investigated.

Methods

We examined Rab18 expression in gastric cancer tissues using immunohistochemistry. We used SNU-1 and AGS cell lines for plasmid and siRNA transfection respectively. MTT, colony formation assay, cell cycle analysis, matrigel invasion, wound healing assay, AnnexinV/PI analysis and western blotting were used to examine the biological effect and mechanism of Rab18 in gastric cancer cell lines.

Results

Rab18 protein expression was upregulated in gastric cancer tissues and this correlated with advanced stage and poor prognosis. Rab18 overexpression promoted proliferation in vitro and in vivo.

SUBMITTER: Wu B 

PROVIDER: S-EPMC6225851 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature