RNA sequencing identifies novel non-coding RNA and exon-specific effects associated with cigarette smoking.

ABSTRACT:

Background

Cigarette smoking is the leading modifiable risk factor for disease and death worldwide. Previous studies quantifying gene-level expression have documented the effect of smoking on mRNA levels. Using RNA sequencing, it is possible to analyze the impact of smoking on complex regulatory phenomena (e.g. alternative splicing, differential isoform usage) leading to a more detailed understanding of the biology underlying smoking-related disease.

Methods

We used whole-blood RNA sequencing to describe gene and exon-level expression differences between 229 current and 286 former smokers in the COPDGene study. We performed differential gene expression and differential exon usage analyses using the voom/limma and DEXseq R packages. Samples from current and former smokers wer

SUBMITTER: Parker MM 

PROVIDER: S-EPMC6225866 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature