Epigenetic regulation of HOTAIR in advanced chronic myeloid leukemia.

ABSTRACT:

Purpose

Chronic myeloid leukemia (CML) accounts for ~10% of leukemia cases, and its progression involves epigenetic gene regulation. This study investigated epigenetic regulation of HOTAIR and its target microRNA, miR-143, in advanced CML.

Patients and methods

We first isolated bone marrow mononuclear cells from 70 patients with different phases of CML and from healthy donors as normal control; we also cultured K562 and KCL22 cells, treated with demethylation drug; MTT assay, flow cytometry, quantitative real-time polymerase chain reaction (qPCR), methylation-specific polymerase chain reaction (MSP), Western blot, luciferase assay, RNA pull-down assay and RNA-binding protein immunoprecipitation (RIP) assay were performed.

Result

As measured by qPCR, HOTAIR expression

SUBMITTER: Li Z 

PROVIDER: S-EPMC6225916 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature