Stage-specific modulation of antimullerian hormone promotes primate follicular development and oocyte maturation in the matrix-free three-dimensional culture.

ABSTRACT:

Objective

To study whether follicular growth and oocyte maturation can be improved by antimüllerian hormone (AMH) modulation at specific stages of follicular development.

Design

Primary and secondary follicles were cultured in a matrix-free system and were assigned to the control group and the group with AMH supplementation during the preantral stage and neutralizing AMH antibody addition during the antral stage.

Setting

National primate research center.

Animal(s)

Adult, female rhesus macaques (Macaca mulatta).

Intervention(s)

None.

Main outcome measure(s)

Follicle survival, growth, steroid and paracrine factor production, and oocyte competence were evaluated. Follicles were assessed for expression of genes that are critical for gonadotropin signa

SUBMITTER: Xu J 

PROVIDER: S-EPMC6226025 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature