The helicase Ded1p controls use of near-cognate translation initiation codons in 5' UTRs.

ABSTRACT: The conserved and essential DEAD-box RNA helicase Ded1p from yeast and its mammalian orthologue DDX3 are critical for the initiation of translation¹. Mutations in DDX3 are linked to tumorigenesis^2-4 and intellectual disability⁵, and the enzyme is targeted by a range of viruses⁶. How Ded1p and its orthologues engage RNAs during the initiation of translation is unknown. Here we show, by integrating transcriptome-wide analyses of translation, RNA structure and Ded1p-RNA binding, that the effects of Ded1p on the initiation of translation are connected to near-cognate initiation codons in 5' untranslated regions. Ded1p associates with the translation pre-initiation complex at the mRNA entry channel and repressing the activity of Ded1p leads to the acc

SUBMITTER: Guenther UP 

PROVIDER: S-EPMC6226265 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature