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Characterization of a thermo-adapted strain of Zymomonas mobilis for ethanol production at high temperature.


ABSTRACT: A thermo-adapted strain of Zymomonas mobilis designated ZM AD41 that capable of growth and ethanol production at high temperature was obtained using the thermal stress adaptation technique. This thermo-adapted strain exhibited approximately 1.8- and 27-fold higher growth rate than the wild-type at 39 °C and 41 °C, respectively. It was more resistant to stress induced by acetic acid at 200 mM and hydrogen peroxide (H2O2) at 0.4 mM and produced approximately 1.8- and 38.6-fold higher ethanol concentrations than the wild-type at 39 °C and 41 °C, respectively. Moreover, it had better sedimentation performance during ethanol fermentation at high temperature than the wild-type. Based on the growth performance, heat, acetic acid and H2O2 stress treatments, sedimentation characteristics, and ethanol fermentation capability, Z. mobilis ZM AD41 was a good candidate for ethanol production at high temperature.

SUBMITTER: Samappito J 

PROVIDER: S-EPMC6226418 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

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Characterization of a thermo-adapted strain of <i>Zymomonas mobilis</i> for ethanol production at high temperature.

Samappito Jatupat J   Yamada Mamoru M   Klanrit Preekamol P   Thanonkeo Pornthap P  

3 Biotech 20181109 11


A thermo<b>-</b>adapted strain of <i>Zymomonas mobilis</i> designated ZM AD41 that capable of growth and ethanol production at high temperature was obtained using the thermal stress adaptation technique. This thermo<b>-</b>adapted strain exhibited approximately 1.8<b>-</b> and 27<b>-</b>fold higher growth rate than the wild<b>-</b>type at 39 °C and 41 °C, respectively. It was more resistant to stress induced by acetic acid at 200 mM and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) at 0.4 mM an  ...[more]

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