The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme.

ABSTRACT: Atmospheric pressure cold plasma (ACP) is introduced as a useful tool in a variety of biological applications. Proteins are the most abundant macromolecules in living systems with a central role in all biological processes. These organic molecules are modified by ACP exposure that is responsible for many of ACP's biological effects. This study evaluated the effect of ACP on the production of recombinant phytase in yeast Pichia pastoris (P. pastoris) as well as the structure and function of the phytase enzyme. The results indicated that yeast cells treated with ACP, directly or indirectly, produced higher amounts of recombinant phytase, which was associated with the time of ACP treatment. The exposure of commercial phytase solution with ACP caused a significant increase in the enzyme activi

SUBMITTER: Farasat M 

PROVIDER: S-EPMC6226467 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature