Integrative analysis of single-cell expression data reveals distinct regulatory states in bidirectional promoters.

ABSTRACT:

Background

Bidirectional promoters (BPs) are prevalent in eukaryotic genomes. However, it is poorly understood how the cell integrates different epigenomic information, such as transcription factor (TF) binding and chromatin marks, to drive gene expression at BPs. Single-cell sequencing technologies are revolutionizing the field of genome biology. Therefore, this study focuses on the integration of single-cell RNA-seq data with bulk ChIP-seq and other epigenetics data, for which single-cell technologies are not yet established, in the context of BPs.

Results

We performed integrative analyses of novel human single-cell RNA-seq (scRNA-seq) data with bulk ChIP-seq and other epigenetics data. scRNA-seq data revealed distinct transcription states of BPs that were previously not r

SUBMITTER: Behjati Ardakani F 

PROVIDER: S-EPMC6230222 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature