Specificity of the IgG antibody response to Plasmodium falciparum, Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale MSP1₁₉ subunit proteins in multiplexed serologic assays.

ABSTRACT:

Background

Multiplex bead assays (MBA) that measure IgG antibodies to the carboxy-terminal 19-kDa sub-unit of the merozoite surface protein 1 (MSP1₁₉) are currently used to determine malaria seroprevalence in human populations living in areas with both stable and unstable transmission. However, the species specificities of the IgG antibody responses to the malaria MSP1₁₉ antigens have not been extensively characterized using MBA.

Methods

Recombinant Plasmodium falciparum (3D7), Plasmodium malariae (China I), Plasmodium ovale (Nigeria I), and Plasmodium vivax (Belem) MSP1₁₉ proteins were covalently coupled to beads for MBA. Threshold cut-off values for the assays were estimated using sera from US citizens with no history of foreign travel and

SUBMITTER: Priest JW 

PROVIDER: S-EPMC6230236 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature