Prevention of ESKAPE pathogen biofilm formation by antimicrobial peptides WLBU2 and LL37.

ABSTRACT:

Objectives

Bacterial biofilm-dependent infections (e.g. cystic fibrosis, surgical sites, and medical implants) are associated with enhanced drug-resistance and are thus difficult to eradicate. The goal of this study was to systematically compare three distinct classes of antimicrobial peptides (AMPs) that include the clinically used antibiotic colistin, the natural AMP LL37, the engineered cationic-AMP WLBU2, and four commonly used antibiotics with different bactericidal mechanisms (tobramycin, ciprofloxacin, ceftazidime, and vancomycin) for biofilm prevention properties.

Methods

Using biofilm-prevention assays, we detected bacterial biomass post-attachment in subinhibitory concentrations (1/3 of the minimum inhibitory concentration [MIC]) for each AMP by the crystal violet

SUBMITTER: Lin Q 

PROVIDER: S-EPMC6230315 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature