Information-Theoretic Approach and Fundamental Limits of Resolving Two Closely Timed Neuronal Spikes in Mouse Brain Calcium Imaging.

ABSTRACT:

Objective

Although optical imaging of neurons using fluorescent genetically encoded calcium sensors has enabled large-scale in vivo experiments, the sensors' slow dynamics often blur closely timed action potentials into indistinguishable transients. While several previous approaches have been proposed to estimate the timing of individual spikes, they have overlooked the important and practical problem of estimating interspike interval (ISI) for overlapping transients.

Methods

We use statistical detection theory to find the minimum detectable ISI under different levels of signal-to-noise ratio (SNR), model complexity, and recording speed. We also derive the Cramer-Rao lower bounds (CRBs) for the problem of ISI estimation. We use Monte-Carlo simulations with biologically deriv

SUBMITTER: Soltanian-Zadeh S 

PROVIDER: S-EPMC6230443 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature