Ontology highlight
ABSTRACT:
SUBMITTER: Webster GR
PROVIDER: S-EPMC6230950 | biostudies-literature | 2018 Dec
REPOSITORIES: biostudies-literature
Webster Gina R GR van Dolleweerd Craig C Guerra Thais T Stelter Szymon S Hofmann Sven S Kim Mi-Young MY Teh Audrey Y-H AY Diogo Gil Reynolds GR Copland Alastair A Paul Mathew J MJ Hart Peter P Reljic Rajko R Ma Julian K-C JK
Plant biotechnology journal 20180721 12
In this study, a strategy based on polymeric immunoglobulin G scaffolds (PIGS) was used to produce a vaccine candidate for Mycobacterium tuberculosis. A genetic fusion construct comprising genes encoding the mycobacterial Ag85B antigen, an immunoglobulin γ-chain fragment and the tailpiece from immunoglobulin μ chain was engineered. Expression was attempted in Chinese Hamster Ovary (CHO) cells and in Nicotiana benthamiana. The recombinant protein assembled into polymeric structures (TB-PIGS) in N ...[more]