Chimeric 3' flanking regions strongly enhance gene expression in plants.

ABSTRACT: Plants represent a promising platform for the highly scalable production of recombinant proteins. Previously, we identified the tobacco extensin terminator lacking its intron as an element that reduced transcript read-through and improved recombinant protein production in a plant-based system. In this study, we systematically compared nonreplicating plant expression vectors containing over 20 commonly used or newly identified terminators from diverse sources. We found that eight gene terminators enhance reporter gene expression significantly more than the commonly used 35S and NOS terminators. The intronless extensin terminator provided a 13.6-fold increase compared with the NOS terminator. Combining terminators in tandem produced large synergistic effects, with many combinations providing

SUBMITTER: Diamos AG 

PROVIDER: S-EPMC6230951 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature